Phosphoric Acid → D-2-Aminohexano-6-lactam

HRID 2222060

反応式

HRID 2222060 の反応式
C1N(CCO)CCN(CCS(O)(=O)=O)C1.[OH-].[Na+].CC1C(O)=C(C=O)C(COP(O)(O)=O)=CN=1.[CH2:34]1[CH2:41][C@H:40]([NH2:42])[C:38](=[O:39])[NH:37][CH2:36][CH2:35]1.OP(O)(O)=O>C1(C)C=CC=CC=1>[CH2:34]1[CH2:41][C@@H:40]([NH2:42])[C:38](=[O:39])[NH:37][CH2:36][CH2:35]1.[CH2:34]1[CH2:41][C@H:40]([NH2:42])[C:38](=[O:39])[NH:37][CH2:36][CH2:35]1

参加物

7

反応物 4

HCID 1004Phosphoric AcidHCID 1051Pyridoxal 5'-phosphateHCID 440599L-2-aminohexano-6-lactamHCID 86606154HEPES NaOH

生成物 2

HCID 439823D-2-Aminohexano-6-lactamHCID 440599L-2-aminohexano-6-lactam

試薬・触媒・溶媒 1

HCID 1140Toluene溶媒

実験手順

Then 100 mg of these lyophilized cells from each monoculture were added to a reaction mixture consisting of 10 mM HEPES-NaOH buffer (pH 7.7), toluene (2,5 v/v %), pyridoxal-5-phosphate (20 μM), and L-α-amino-ε-caprolactam (2.5 m %). Chemical blanks (with the assay mixture without the lyophilized cells) were used as a negative control. All reaction mixtures (including the blanks) were incubated for approximately 72 hours at 40° C. before the reaction was stopped by the addition of 9 volumes of 1 M H3PO4. After removal of the cells by filtration through a 0.45 μM filter, these reaction mixtures were analyzed by the HPLC method described above to determine the concentration of D- and L-α-amino-ε-caprolactam. Finally, monocultures that formed D-α-amino-ε-caprolactam from the L-α-amino-ε-caprolactam substrate were sent to the NCIMB for strain determination. Nb. no D-α-amino-ε-caprolactam could be determined in the chemical blanks.

後処理

  1. additionThen 100 mg of these lyophilized cells from each monoculture were added to a reaction mixture
  2. customAll reaction mixtures (
  3. customAfter removal of the cells
  4. filtrationby filtration through a 0.45 μM
  5. filtrationfilter
  6. customthese reaction mixtures

出典と根拠

ORDレコード
ord-2c1aa305598d45a992ce5851134dedfa
出典データセット
uspto-grants-2008_05
特許番号
US07371555B2

関連ノート

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