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Glycerol → Bistris
関与物質
| 役割 | 名称 | HCID | 仕込み量 |
|---|---|---|---|
| 反応物 | Glycerol | HCID 753 | — |
| 反応物 | Tris(hydroxymethyl)aminomethane | HCID 6503 | — |
| 反応物 | Bromophenol Blue | HCID 8272 | — |
| 反応物 | D-1,4-Dithiothreitol | HCID 446094 | — |
| 反応物 | Sodium Lauryl Sulfate | HCID 3423265 | — |
| 生成物 | Bistris | HCID 81462 | 10% |
反応手順
Protein samples were prepared for SDS-PAGE by diluting 1:1 with 2× denaturing buffer (40 μg/ml SDS, 20% glycerol, 30 μg/ml DTT and 10 μg/ml bromophenol blue in 125 mM Tris, pH 6.8) and boiling for 2 min. Pre-cast Nu-PAGE 10% Bis-Tris protein gels were obtained from Invitrogen (Carlsbad, Calif.). Each lane was loaded with a maximum of 10 μl of sample, and run for 50 min at 200V. After electrophoresis was complete, the gel was stained with Invitrogen SafeStain (1 hour in staining solution, then washed overnight with water).
情報源と証拠
| DOI | 10.6084/m9.figshare.5104873.v1 |
|---|---|
| 特許番号 | US08209132B2 |
| 出典データセット | uspto-grants-2012_06 |
| ORD ID | ord-4d80f4b2e6004f738223602995764cd5 |
| 出典メモ | uspto-grants-2012_06 |