反応式
C([NH:4][CH:5]([C:10]([OH:12])=[O:11])[CH2:6][CH2:7][S:8][CH3:9])(=O)C>P([O-])([O-])([O-])=O.[Co](Cl)Cl>[NH2:4][C@H:5]([C:10]([OH:12])=[O:11])[CH2:6][CH2:7][S:8][CH3:9]参加物
4
実験手順
To test the bound aminoacylase activity, 100 mg of the charged carrier were carefully shaken for 2 minutes at room temperature in 3 ml of 0.1 molar phosphate buffer solution (pH 7.6) containing 10 mg/ml of N-acetyl-D,L-methionine and 0.36 mg of cobalt chloride (CoCl2 ·6 H2O). The amount of L-methionine formed was then found by reaction with L-aminoacid-oxidase and determination of the resulting hydrogen peroxide with peroxidase by UV-spectrophotometric measurement of o-dianisidine (in the oxidized, ie. quinoid, form) (Methods in Enzymology, Vol. II).
出典と根拠
- ORDレコード
- ord-ae3e76166ad04b4d90e605eef355d101
- 出典データセット
- uspto-grants-1980_11
- 特許番号
- US04235973
関連ノート
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