Indole-3-pyruvic acid → L-Tryptophan

HRID 2257454

反応式

HRID 2257454 の反応式
[NH:1]1[C:9]2[C:4](=[CH:5][CH:6]=[CH:7][CH:8]=2)[C:3]([CH2:10][C:11](=O)[C:12]([OH:14])=[O:13])=[CH:2]1.CC1C(O)=C(CN)C(COP(O)(O)=O)=C[N:18]=1.P([O-])([O-])([O-])=O>O>[NH2:18][C@H:11]([C:12]([OH:14])=[O:13])[CH2:10][C:3]1[C:4]2[C:9](=[CH:8][CH:7]=[CH:6][CH:5]=2)[NH:1][CH:2]=1

参加物

5

反応物 3

HCID 803Indole-3-pyruvic acidHCID 1053Pyridoxamine 5'-phosphateHCID 1061Phosphate

生成物 1

HCID 6305L-Tryptophan

試薬・触媒・溶媒 1

HCID 962Water溶媒

反応条件

温度
37 °C

実験手順

To a solution containing 20.3 g (100mmol) of indole-3-pyruvic acid and 26.5 g (100 mmol) of pyridoxamine-5-phosphate in 1000 ml of distilled water buffered at pH 6.5 with 0.5M phosphate buffer and maintained at 37° C. is added 50 mg of the monoclonal antibodies prepared according to Example 15. The reaction mixture is gently agitated for 2 hours. The monoclonal antibodies are then recovered by ultrafiltration. Dialysis of the reaction mixture followed by lyophilization yields the product L-tryptophan.

後処理

  1. additionis added 50 mg of the monoclonal antibodies
  2. customprepared
  3. filtrationThe monoclonal antibodies are then recovered by ultrafiltration
  4. customDialysis of the reaction mixture

出典と根拠

ORDレコード
ord-56cee0b26948462bbbd089f8b109a103
出典データセット
uspto-grants-1997_08
特許番号
US05658752

関連ノート

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