Phosphate → HCID 23276146、化合物

HRID 2287198ノート

反応式

HRID 2287198 の反応式
[Na+].[OH:2][C:3]1[C:16]2[C:15](=[O:17])[CH:14]=[C:13]([C:18]([O-:20])=[O:19])[O:12][C:11]=2[C:10]([CH2:21][CH2:22][CH3:23])=[C:9]2[C:4]=1[CH2:5][CH2:6][CH2:7][CH2:8]2.P([O-])([O-])([O-])=[O:25].C1C=[N+]([C@H]2O[C@@H](COP(OP(OC[C@H]3O[C@@H](N4C5N=CN=C(N)C=5N=C4)[C@H](OP(O)(O)=O)[C@@H]3O)(O)=O)([O-])=O)[C@H](O)[C@@H]2O)C=C(C(N)=O)C=1.C(OP(O)(O)=O)[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O>>[OH:2][C:3]1[C:16]2[C:15](=[O:17])[CH:14]=[C:13]([C:18]([OH:20])=[O:19])[O:12][C:11]=2[C:10]([CH2:21][CH2:22][CH3:23])=[C:9]2[C:4]=1[CH2:5][CH:6]([OH:25])[CH2:7][CH2:8]2

参加物

5

反応物 4

HCID 1061PhosphateHCID 439427Beta-D-Glucose 6-phosphateHCID 23668092HCID 23668092、化合物HCID 71717786[[(2R,3R,4R,5R)-5-(6-aminopurin-9-yl)-3-hydroxy-4-phosphonooxyoxolan-2-yl]methoxy-hydroxyphosphoryl] [(2S,3R,4S,5S)-5-(3-carbamoylpyridin-1-ium-1-yl)-3,4-dihydroxyoxolan-2-yl]methyl phosphate

生成物 1

HCID 23276146HCID 23276146、化合物

実験手順

A mixture of 5 parts 6,7,8,9-tetrahydro-5-hydroxy-4-oxo-10-propyl-4H-naphtho[2,3-b]pyran-2-carboxylic acid sodium salt, 4 parts of 25% w/v supernatant liver fraction, 10 parts of phosphate buffer pH 7.4, one part of nicotinamide adenine dinucleotide phosphate and one part of glucose-6-phosphate was incubated in a shaking incubator at 37° C. for 30 minutes. The reaction was stopped and protein was precipitated by the addition of 5 parts of 2 N hydrochloric acid. The reaction mixture was extracted three times with 100 parts of diethyl ether and after being dried over anhydrous sodium sulphate the extracts were evaporated to dryness at room temperature in a current of air. The 5,7-dihydroxy-6,7,8,9-tetrahydro-4-oxo-10-propyl-4H-naphtho[2,3-b]pyran-2-carboxylic acid and the other dihydroxy compounds identified in Example 1 were isolated by the preparative layer chromatography technique described in Example 1.

後処理

  1. customprotein was precipitated by the addition of 5 parts of 2 N hydrochloric acid
  2. extractionThe reaction mixture was extracted three times with 100 parts of diethyl ether
  3. dry with materialafter being dried over anhydrous sodium sulphate the extracts
  4. customwere evaporated to dryness at room temperature in a current of air
  5. customwere isolated by the preparative layer chromatography technique

出典と根拠

ORDレコード
ord-86045fdefbd144d2ba3f85d4bf63eb2e
出典データセット
uspto-grants-1981_05
特許番号
US04269854

反応ノート

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