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Phosphate → L-(-)-Ornithine
関与物質
| 役割 | 名称 | HCID | 仕込み量 |
|---|---|---|---|
| 反応物 | Phosphate | HCID 1061 | — |
| 反応物 | L-Ascorbic Acid | HCID 54670067 | — |
| 反応物 | HCID 178418600 | HCID 178418600 | — |
| 反応物 | HCID 178512957 | HCID 178512957 | — |
| 触媒 | Manganese Sulfate | HCID 24580 | — |
| 溶媒 | Water | HCID 962 | — |
| 溶媒 | Acetonitrile | HCID 6342 | — |
| 生成物 | L-(-)-Ornithine | HCID 6262 | — |
反応手順
1.74 g DL-arginine (50:50) were dissolved in 10 ml water and mixed with 0.21 mg manganese sulfate.H2O, 0.11 mg ascorbic acid and 0.5 mg calf liver L-arginase (Boeringer Mannheim, 97 U/mg. The progress of the reaction was followed with HPLC (amino column; mobile solvent 65:35 acetonitrile:phosphate buffer (pH 6.4). After 6 hours at 25° C., 50% of the original amount of arginine had reacted and a corresponding amount of ornithine had been formed (conversion 100%).
情報源と証拠
| DOI | 10.6084/m9.figshare.5104873.v1 |
|---|---|
| 特許番号 | US05591613 |
| 出典データセット | uspto-grants-1997_01 |
| ORD ID | ord-d0f7a5dd9a9e4a499ca06ed3a43f1697 |
| 出典メモ | uspto-grants-1997_01 |