関与物質
| 役割 | 名称 | HCID | 仕込み量 |
|---|
| 反応物 | Potassium Chloride | HCID 4873 | — |
| 反応物 | 5'-Atp | HCID 5957 | — |
| 反応物 | Acrylamide | HCID 6579 | — |
| 反応物 | 4-(2-Hydroxyethyl)-1-piperazineethanesulfonic acid | HCID 23831 | — |
| 反応物 | N-Acetyl-Neuraminic Acid | HCID 439197 | — |
| 反応物 | beta-D-galactosyl-(1->4)-N-acetyl-D-glucosamine | HCID 9800166 | — |
| 反応物 | CMP-NeuAc | HCID 23679065 | — |
| 溶媒 | Water | HCID 962 | — |
| 生成物 | GlyTouCan:G87605GW | HCID 131718308 | 97% |
反応手順
NeuAc (0.92 g, 3 mmol), Galβ4GlcNAc (1.1 g, 3 mmol), CMP (0.1 g, 30 μmol), ATP (16 mg, 3 μmol), PEP (2.8 g, 6 mmol), MgCl2.6H2O (0.61 g, 3 mmol), MnCl2.4H2O (0.15 g, 0.8 mmol), KCl (0.22 g, 3 mmol), NMK or MK (450 U), PK (6,000 U), PPase (300 U), CMP-NeuAc synthetase (24 U), and Siaα2,6Gal sialyl transferase (4 U) were mixed with 150 ml of HEPES buffer (0.2M, pH 7.5) to form a reaction mixture and the reaction mixture maintained under argon at about 25° C. for about 48 hours. After the disappearance of NeuAc (determined by thin-layer chromatography) the reaction mixture was reduced in volume to 20 ml by lyophilization and the lyophilized reaction mixture applied to a Bio Gel P2 (200-400 mesh) column with water as the mobile phase. The trisaccharide-containing fractions were eluted, collected and lyophilized to give pure Neuα2,6Galβ1,4GlcNAc in 97 percent yield.
情報源と証拠
| DOI | 10.6084/m9.figshare.5104873.v1 |
|---|
| 特許番号 | US05593887 |
|---|
| 出典データセット | uspto-grants-1997_01 |
|---|
| ORD ID | ord-43871dab186d42b3850b75c2d43cf09b |
|---|
| 出典メモ | uspto-grants-1997_01 |
|---|
