HCID 605502、化合物 → HCID 15612355、化合物

HRID 2371367ノート

反応式

HRID 2371367 の反応式
[CH3:1][O:2][C:3]([CH:5]1[C:12](=[O:13])[CH:11](C(OC)=O)[CH:10]2[CH:6]1[CH2:7][C:8](=[O:18])[CH2:9]2)=[O:4]>CO.P([O-])([O-])([O-])=O>[CH3:1][O:2][C:3]([CH:5]1[C:12](=[O:13])[CH2:11][CH:10]2[CH:6]1[CH2:7][C:8](=[O:18])[CH2:9]2)=[O:4]

参加物

4

反応物 1

HCID 605502HCID 605502、化合物

生成物 1

HCID 15612355HCID 15612355、化合物

試薬・触媒・溶媒 2

HCID 887Methanol溶媒HCID 1061Phosphate溶媒

実験手順

One gram of 2,4-bismethoxycarbonylbicyclo[3.3.0]octane-3,7-dione is dissolved in 20 ml of methanol and combined with a solution of 2.5 g of α-chymotrypsin in 150 ml of 0.1-molar phosphate buffer pH 7. The solution is extracted with methyl isobutyl ketone after 22 hours of agitation at 28° C.; the extract is concentrated under vacuum, and the remaining residue is chromatographed over a silica gel column (gradient hexane/ethyl acetate), thus obtaining 610 mg of optically pure 2-methoxycarbonylbicyclo[3.3.0]octane-3,7-dione as an oily liquid.

後処理

  1. extractionThe solution is extracted with methyl isobutyl ketone after 22 hours of agitation at 28° C.
  2. concentrationthe extract is concentrated under vacuum
  3. customthe remaining residue is chromatographed over a silica gel column (gradient hexane/ethyl acetate)

出典と根拠

ORDレコード
ord-c9df848a40d0432db9a2709eb5c55621
出典データセット
uspto-grants-1992_03
特許番号
US05093250

反応ノート

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