関与物質
| 役割 | 名称 | HCID | 仕込み量 |
|---|
| 反応物 | BW284C51 | HCID 1338 | — |
| 反応物 | Decamethonium | HCID 2968 | — |
| 反応物 | Edrophonium | HCID 3202 | — |
| 反応物 | 5,5'-Dithiobis(2-nitrobenzoic Acid) | HCID 6254 | — |
| 反応物 | Acetylthiocholine iodide | HCID 74629 | — |
| 反応物 | D-1,4-Dithiothreitol | HCID 446094 | — |
| 反応物 | (-)-Isoselagine | HCID 907504 | — |
| 反応物 | 1,2,3,4-Tetrahydro-9-acridinamine hydrochloride hydrate (1:?:?) | HCID 6420002 | — |
| 反応物 | m-(N,N,N-trimethylammonio)trifluoromethylacetophenone | HCID 69751146 | — |
| 生成物 | Acrylodan | HCID 104901 | — |
反応手順
Inhibitors and Substrates-Acetylthiocholine iodide, 5,5′-dithiobis(2-nitrobenzoic acid) (Ellman's reagent), dithiothreitol, tacrine (9-amino-1,2,3,4-tetrahydroacridine hydrochloride hydrate), BW284c51, decamethonium, and edrophonium were purchased from Sigma. m-(N,N,N-trimethylammonio)trifluoromethylacetophenone (TFK+) and (−)huperzine A were purchased from Calbiochem. Acrylodan was obtained from Molecular Probes (Eugene, Oreg.). Fasciculin 2 (purified from the venom of Dendroaspis angusticeps) was a gift of Dr. Pascale Marchot (University of Marseille, France). Drs. Yacov Ashani and Bhupendra P. Doctor (Walter Reed Army Research Center, Washington, D.C.) kindly provided 7-((methylethoxy) phosphinyl)-oxyl)-1-methylquinolinium iodide (MEPQ) and procainamide-linked Sepharose CL-4B resin. m-tert-Butyl trifluoromethylacetophenone (TFK+) was synthesized as described (21) and kindly provided by Dr. Daniel Quinn, University of Iowa, Iowa City, Iowa. All other chemicals were of the highest grade commercially available.
情報源と証拠
| DOI | 10.6084/m9.figshare.5104873.v1 |
|---|
| 特許番号 | US08642273B2 |
|---|
| 出典データセット | uspto-grants-2014_02 |
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| ORD ID | ord-85a0f7713e78471f8f2b54ff219b3f42 |
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| 出典メモ | uspto-grants-2014_02 |
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