Ammonia → 3-Amino-4-thiophen-2-yl-5,6,7,8-tetrahydro-1-thia-7,9-diaza-cyclopenta[b]naphthalene-2-carboxylic acid amide

反応式

C(OC([N:8]1[CH2:17][C:16]2[N:15]=[C:14]3[S:18][C:19]([C:22](=[O:24])[NH2:23])=[C:20]([NH2:21])[C:13]3=[C:12]([C:25]3[S:26][CH:27]=[CH:28][CH:29]=3)[C:11]=2[CH2:10][CH2:9]1)=O)(C)(C)C.C(Cl)(=O)C.N>CO>[NH2:21][C:20]1[C:13]2[C:14](=[N:15][C:16]3[CH2:17][NH:8][CH2:9][CH2:10][C:11]=3[C:12]=2[C:25]2[S:26][CH:27]=[CH:28][CH:29]=2)[S:18][C:19]=1[C:22]([NH2:23])=[O:24]

参加物

実験手順

To a suspension of 3-amino-2-carbamoyl-4-thiophen-2-yl-5,8-dihydro-6H-1-thia-7,9-diaza-cyclopenta[b]naphthalene-7-carboxylic acid tert-butyl ester (100 mg) in 10 ml of anhydrous methanol under argon 0.5 ml of acetyl chloride was added dropwise, and the resulting mixture was stirred overnight at room temperature. The reaction mixture was neutralized with 7N ammonia in methanol and evaporated to dryness under vacuum. The residue was purified by silica gel column (chloroform/methanol, 30:1) to give a yellow solid. 1H-NMR (300 MHz, DMSO-d6): δ 7.88 (d, J=5.1 Hz, 1H), 7.28 (dd, J=3.6, 5.1 Hz, 1H), 7.16 (d, J=3.6 Hz, 1H), 7.01 (brs, 2H), 5.64 (brs, 2H), 4.84 (brs, 1H), 3.16 (s, 2H), 2.94 (m, 2H), 1.86 (m, 2H). ES MS m/z 331 (M+H)+, 329 (M−H)−.