反応式
CCCCC[C@H](O)/C=C/[C@@H]1[C@@H](C/C=C\CCC[C:20]([OH:22])=[O:21])[C@H]2OO[C@@H]1C2.C(O)(=[O:46])CCC/C=C\C/C=C\C/C=C\C/C=C\CCCCC.C[NH:49][CH2:50][C@H:51](O)[C:52]1[CH:53]=[CH:54][C:55]([OH:59])=[C:56](O)[CH:57]=1.C(N)CN([N:67]([OH:70])N=O)CCN>P([O-])([O-])([O-])=O.CC1C2[N-]C(=CC3[N-]C(C=C4N=C(C=C5N=C(C=2)C(C=C)=C5C)C(C=C)=C4C)=C(C)C=3CCC(O)=O)C=1CCC(O)=O.O.[Fe+2]>[N+:67]([NH:49][C@H:50]([C:20]([OH:22])=[O:21])[CH2:51][C:52]1[CH:53]=[CH:54][C:55]([OH:59])=[CH:56][CH:57]=1)([O-:70])=[O:46]参加物
7
実験手順
Reaction mixture in 100 mM phosphate buffer, pH 7.4, (total volume, 200 μl) was incubated at 37° C. for 30 minutes. The reaction mixture contained a combination of cytochrome c (40 μg), ovine prostaglandin H2 synthase form 2 (104 units), arachidonic acid (590 μM), epinephrine (31 μg), hematin (1 μM), and DETA NONOate, an NO donor, (300 μM) as indicated in FIG. 2. After incubation, the proteins were separated by SDS-PAGE followed by Western blot analysis with an alkaline phosphatase system using monoclonal antibodies produced against nitrotyrosine.
後処理
- customAfter incubation, the proteins were separated by SDS-PAGE
出典と根拠
- ORDレコード
- ord-76199e79b92a4202aea748587ed7142b
- 出典データセット
- uspto-grants-2004_11
- 特許番号
- US06812212B2
関連ノート
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