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Trifluoroacetic acid → Lys-Val-Lys
関与物質
| 役割 | 名称 | HCID | 仕込み量 |
|---|---|---|---|
| 反応物 | Trifluoroacetic acid | HCID 6422 | — |
| 反応物 | FMOC-L-valine | HCID 688217 | — |
| 反応物 | Fmoc-Lys(Boc)-OH | HCID 2724628 | — |
| 反応物 | (2S)-2-amino-6-(((tert-butoxy)carbonyl)amino)hexanoic acid | HCID 2733283 | — |
| 溶媒 | Diethyl Ether | HCID 3283 | — |
| 生成物 | Lys-Val-Lys | HCID 448140 | — |
反応手順
The protective peptide is built on 1.00 g (0.80 mmol) of H-Lys(Boc)-2-chlorotrityl resin using Fmoc-Val-OH, Fmoc-Lys(Boc)-OH and Palm-OSu. The resin is treated for 30 min with 8 ml of TFA 95% and the solution is instilled in 100 ml of Et2O. The precipitate is sucked off, washed and purified by prep. HPLC after drying. The substance is diluted in 30 ml of dioxan:water=4:6, treated overnight with 2.0 g of BioRad resin (acetate form), filtered, rotated and lyophilized. Yield: 110 mg (0.15 mmol, 19%)
情報源と証拠
| DOI | 10.6084/m9.figshare.5104873.v1 |
|---|---|
| 特許番号 | US07863417B2 |
| 出典データセット | uspto-grants-2011_01 |
| ORD ID | ord-73c73a56b2d444b7857252e72f7b484d |
| 出典メモ | uspto-grants-2011_01 |