5,5'-Dithiobis(2-nitrobenzoic Acid) → N-[4-(5-carboxypentylcarbamoyl)-cyclohexylmethyl]-2-(hydroxy)ethyl thiosuccinimide

HRID 260223

反応式

HRID 260223 の反応式
SC([OH:4])C.C1C(CN2C(=O)C=CC2=O)CCC(C(ON2C(=O)CCC2=O)=O)C1.C([O-])(=O)CCCCC.C1C(SSC2C=CC([N+]([O-])=O)=C(C(O)=O)C=2)=CC(C(O)=O)=C([N+]([O-])=O)C=1.[C:63]([CH2:66][CH2:67][CH2:68][CH2:69][CH2:70][NH:71][C:72]([CH:74]1[CH2:79][CH2:78][CH:77]([CH2:80][N:81]2[C:85](=[O:86])[CH2:84][CH:83]([CH2:87][CH2:88]C(O)=O)[C:82]2=[S:92])[CH2:76][CH2:75]1)=[O:73])([OH:65])=[O:64]>>[C:63]([CH2:66][CH2:67][CH2:68][CH2:69][CH2:70][NH:71][C:72]([CH:74]1[CH2:79][CH2:78][CH:77]([CH2:80][N:81]2[C:85](=[O:86])[CH2:84][CH:83]([CH2:87][CH2:88][OH:4])[C:82]2=[S:92])[CH2:76][CH2:75]1)=[O:73])([OH:65])=[O:64]

参加物

5

反応物 4

HCID 62545,5'-Dithiobis(2-nitrobenzoic Acid)HCID 4713212Mercapto-ethanolHCID 86749545N-[4-(5-carboxypentylcarbamoyl)-cyclohexylmethyl]-2-(carboxy)ethyl thiosuccinimideHCID 131719433SMCC caproate

生成物 1

HCID 86749544N-[4-(5-carboxypentylcarbamoyl)-cyclohexylmethyl]-2-(hydroxy)ethyl thiosuccinimide

実験手順

Mercaptoethanol (0.0045 ml of 1.43M solution in dimethylformamide, 6.4×10-6 mol) was added to SMCC-caproate (0.016 ml, 6.4×10-6 mol) in pyridine (see Example 6). The solution was gassed with argon and the tube sealed. After 12 hr at room temperature, no thiol remained (as determined by reaction with DTNB) and the solvents were removed in vacuo. The residue was dissolved in 0.03 ml of 0.2M potassium phosphate, pH 7, to make a 213 mM solution of the desired compound (see FIG. 1D).

後処理

  1. customthe tube sealed
  2. customthe solvents were removed in vacuo
  3. dissolutionThe residue was dissolved in 0.03 ml of 0.2M potassium phosphate

出典と根拠

ORDレコード
ord-129d7d3354f0455c9470b7afcd62d517
出典データセット
uspto-grants-1996_06
特許番号
US05525524

関連ノート

読み込み中…