Nitrophenylgalactosides → Indole
反応式
[CH:1]1[CH:6]=[C:5]([N+:7]([O-])=O)[C:4](O[C@@H]2O[C@H](CO)[C@H](O)[C@H](O)[C@H]2O)=[CH:3][CH:2]=1.O[CH:23]1O[C@H](CO)[C@@H](O[C@@H]2O[C@H](CO)[C@H](O)[C@H](O)[C@H]2O)[C@H](O)[C@H:24]1O>>[NH:7]1[C:5]2[C:4](=[CH:3][CH:2]=[CH:1][CH:6]=2)[CH:24]=[CH:23]1参加物
3
実験手順
Principle of Step 1: ONPG substituting for lactose is metabolized with β-galactosidase. At 44.5° C. E. coli grow and utilize ONPG and produce indole. β-galactoside turns the medium yellow. In the yellow colored medium, both fecal coliforms and E. coli are present. In samples which do not have a yellow color, there are no fecal coliforms and E. coli.
後処理
- customAt 44.5° C
出典と根拠
- ORDレコード
- ord-eb280669c71847b59bf6ce4570e27668
- 出典データセット
- uspto-grants-1997_07
- 特許番号
- US05643743
反応ノート
読み込み中…