5-(1-Propynyl)-2'-deoxyuridine → 5-Propynyluracil

HRID 263402ノート

反応式

HRID 263402 の反応式
[C:1]([C:4]1[C:5](=[O:19])[NH:6][C:7](=[O:18])[N:8]([CH:17]=1)[C@@H]1O[C@H](CO)[C@@H](O)C1)#[C:2][CH3:3]>P([O-])([O-])([O-])=O>[C:1]([C:4]1[C:5](=[O:19])[NH:6][C:7](=[O:18])[NH:8][CH:17]=1)#[C:2][CH3:3]

参加物

3

反応物 1

HCID 4802655-(1-Propynyl)-2'-deoxyuridine

生成物 1

HCID 1318135-Propynyluracil

試薬・触媒・溶媒 1

HCID 1061Phosphate溶媒

実験手順

To a stirred solution of 2'-deoxy-5-propynyluridine (European Patent Specification No. 272065) (20 g, 75 mmol) in aqueous phosphate buffer at pH 6.84 (1250 mL) was added purified E. coli thymidine phosphorylase (10,000 units) (T. A. Krenitsky et al, Biochemistry, 20, 3615, 1981; U.S. Pat. No. 4,381,344) and alkaline phosphatase (10,000 units) [Sigma type VII-S from bovine intestinal mucosa] and the whole mixture was incubated at 37° C. for 24 hours. The resulting white precipitate was filtered, washed with water (3×100 mL), ethanol (2×100 mL), ether (2×100 mL) and dried in vacuo over phosphorus pentoxide to give the title compound.

後処理

  1. custompurified E
  2. filtrationThe resulting white precipitate was filtered
  3. washwashed with water (3×100 mL), ethanol (2×100 mL), ether (2×100 mL)
  4. dry with materialdried in vacuo over phosphorus pentoxide

出典と根拠

ORDレコード
ord-ef09e1a6e55548e096f74a06d24fbec2
出典データセット
uspto-grants-1997_07
特許番号
US05643913

反応ノート

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