関与物質
| 役割 | 名称 | HCID | 仕込み量 |
|---|
| 反応物 | Thiamine Hydrochloride | HCID 6202 | — |
| 反応物 | Potassium Phosphate, Dibasic | HCID 24450 | — |
| 反応物 | Magnesium Sulfate Heptahydrate | HCID 24843 | — |
| 反応物 | Ammonium Chloride | HCID 25517 | — |
| 反応物 | (2R,3S,4R,5R)-2,3,4,5,6-pentahydroxyhexanal | HCID 107526 | — |
| 反応物 | Biotin | HCID 171548 | — |
| 反応物 | Monobasic potassium phosphate | HCID 516951 | — |
| 触媒 | Zinc Sulfate Heptahydrate | HCID 62640 | — |
| 触媒 | Ferrous sulfate heptahydrate | HCID 62662 | — |
| 触媒 | Manganese II sulfate pentahydrate | HCID 22138077 | — |
| 生成物 | L-Glutamine | HCID 5961 | — |
反応手順
Each of the strain ASL-7 and its parent strain ATCC14752 was cultured at 28° C. for 24 hours on the BYG agar medium. One loopful of the cultured cells were inoculated into a 300 ml capacity conical flask charged with 20 ml of a production medium (a medium prepared by dissolving 150 g of glucose, 50 g of ammonium chloride, 0.7 g of dipotassium hydrogenphosphate, 0.7 g of potassium dihydrogenphosphate, 0.5 g of magnesium sulfate heptahydrate, 20 mg of iron sulfate heptahydrate, 20 mg of manganese sulfate pentahydrate, 10 mg of zinc sulfate heptahydrate, 6 μg of biotin, 1 mg of thiamine hydrochloride and 5 g of meat extract in 1 liter of water, adjusting its pH to 7.2 and then adding 50 g of calcium carbonate thereto) and cultured at 28° C. for 96 hours.
情報源と証拠
| DOI | 10.6084/m9.figshare.5104873.v1 |
|---|
| 特許番号 | US07445915B2 |
|---|
| 出典データセット | uspto-grants-2008_11 |
|---|
| ORD ID | ord-a35d2c485bfd472ba49de6dc317d6d95 |
|---|
| 出典メモ | uspto-grants-2008_11 |
|---|
