関与物質
| 役割 | 名称 | HCID | 仕込み量 |
|---|
| 反応物 | NADP nicotinamide-adenine-dinucleotide phosphate | HCID 5886 | — |
| 反応物 | Sodium Hydroxide | HCID 14798 | — |
| 反応物 | (2R,3S,4R,5R)-2,3,4,5,6-pentahydroxyhexanal | HCID 107526 | — |
| 反応物 | Methylamine hydrochloride | HCID 6364545 | — |
| 反応物 | Sodium phenylpyruvate | HCID 23667645 | — |
| 生成物 | N-Methylphenylalanine | HCID 6951135 | 75% |
反応手順
To the resting cells obtained, a reaction solution containing sodium phenylpyruvate in a final concentration of 100 mM, NADP in a final concentration of 0.2 mM, glucose in a final concentration of 100 mM, and methylamine-hydrochloric acid (pH 9) in a final concentration of 700 mM was added to achieve cell turbidity of 20 (absorbance at 660 nm). The reaction was conducted while stirring at 30° C. and adjusting pH to 8 to 9 with 10 N aqueous sodium hydroxide solution. The amount of N-methylphenylalanine produced after 24 hours of reaction was 13.4 g/L (reaction yield=75%).
情報源と証拠
| DOI | 10.6084/m9.figshare.5104873.v1 |
|---|
| 特許番号 | US07452704B2 |
|---|
| 出典データセット | uspto-grants-2008_11 |
|---|
| ORD ID | ord-d18ca109c7f44033868fc43d034e8562 |
|---|
| 出典メモ | uspto-grants-2008_11 |
|---|
