Citric Acid → Thromboxane

HRID 280845

反応式

HRID 280845 の反応式
[CH3:1][CH2:2][CH2:3][CH2:4][CH2:5][C@H:6](O)/[CH:7]=[CH:8]/[C@@H:9]1[C@@H:13]([CH2:14]/[CH:15]=[CH:16]\[CH2:17][CH2:18][CH2:19][C:20](O)=O)[C@H:12]2O[O:24][C@@H:10]1[CH2:11]2.C(O)(=O)CC(CC(O)=O)(C(O)=O)O>CCOCC>[CH3:20][CH2:19][CH2:18][CH2:17][CH2:16][CH2:15][CH2:14][C@@H:13]1[C@@H:9]([CH2:8][CH2:7][CH2:6][CH2:5][CH2:4][CH2:3][CH2:2][CH3:1])[O:24][CH2:10][CH2:11][CH2:12]1

参加物

4

反応物 2

HCID 311Citric AcidHCID 445049Prostaglandin H2

生成物 1

HCID 114873Thromboxane

試薬・触媒・溶媒 1

HCID 3283Diethyl Ether溶媒

実験手順

To a 12×75 mm tube containing [1-14C] PGH2 (2 μg, 0.01 μCi or 0.05 μCi) and inhibitor (1 mM final assay concentration, in 10 μl of ethanol) in 225 μl of phosphate buffer (0.1M, pH 7.5), was added to an aliquot of guinea pig lung microsomes (25 μl, containing 400 μg protein) or an aliquot of human platelet microsomes (25 μl, containing ≅130 g protein). The solution was incubated in a 22° water bath for 2 min.; ether (1 ml) and citric acid (25 μMoles) were added, and the tube plunged into an acetone-dry ice bath. The ether extract was transferred to a 1.0 ml Reacti-vial, and the volume reduced under nitrogen to ≅50 μl.

後処理

  1. concentrationassay concentration
  2. additionin 10 μl of ethanol) in 225 μl of phosphate buffer (0.1M, pH 7.5), was added to an aliquot of guinea pig lung microsomes (25 μl, containing 400 μg protein)
  3. additionan aliquot of human platelet microsomes (25 μl, containing ≅130 g protein)
  4. customwas incubated in a 22°
  5. customfor 2 min.
  6. customthe tube plunged into an acetone-dry ice bath
  7. extractionThe ether extract

出典と根拠

ORDレコード
ord-3fb143d178f5413aa787aa30852b9720
出典データセット
uspto-grants-1986_05
特許番号
US04591594

関連ノート

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