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Kanamycin → L-Phenylalanine
関与物質
| 役割 | 名称 | HCID | 仕込み量 |
|---|---|---|---|
| 反応物 | Kanamycin | HCID 6032 | — |
| 反応物 | L-Leucine | HCID 6106 | — |
| 反応物 | Trisodium citrate | HCID 6224 | — |
| 反応物 | Potassium Phosphate, Dibasic | HCID 24450 | — |
| 反応物 | (2R,3S,4R,5R)-2,3,4,5,6-pentahydroxyhexanal | HCID 107526 | — |
| 反応物 | Monobasic potassium phosphate | HCID 516951 | — |
| 反応物 | HCID 178418600 | HCID 178418600 | — |
| 生成物 | L-Phenylalanine | HCID 6140 | — |
反応手順
The cell-suspension was transferred onto a minimum medium prepared by adding 5 μg/dl kanamycin, 10 mg/dl L-leucine, 10 mg/dl L-leucine, 10 mg/dl L-arginine, 100 mg/dl D, L-m-fluorophenylalanine and 2 g/dl agar to a basal minimum medium of pH 7.2 containing 0.6 g/dl KH2PO4, 1.4 g/dl K2HPO4, 0.2 g/dl (NH4)2SO4, 0.1 g/dl sodium citrate, 0.02 g/dl MgSO4.7H2O, and 0.5 g/dl glucose. After 3 days cultivation at 37° C., two colonies appeared on the agar-medium.
情報源と証拠
| DOI | 10.6084/m9.figshare.5104873.v1 |
|---|---|
| 特許番号 | US04591562 |
| 出典データセット | uspto-grants-1986_05 |
| ORD ID | ord-c3807252a2b846b6b79e083ef66b1ba9 |
| 出典メモ | uspto-grants-1986_05 |