Water → N,N'-Dicyclohexylurea

HRID 32339

反応式

HRID 32339 の反応式
[CH:1]1([N:7]=[C:8]=[N:9][CH:10]2[CH2:15][CH2:14][CH2:13][CH2:12][CH2:11]2)[CH2:6][CH2:5][CH2:4][CH2:3][CH2:2]1.[OH2:16]>CN(C=O)C>[C:8]([NH:7][CH:1]1[CH2:2][CH2:3][CH2:4][CH2:5][CH2:6]1)([NH:9][CH:10]1[CH2:15][CH2:14][CH2:13][CH2:12][CH2:11]1)=[O:16]

参加物

4

反応物 2

HCID 962WaterHCID 10868Dicyclohexylcarbodiimide

生成物 1

HCID 4277N,N'-Dicyclohexylurea

試薬・触媒・溶媒 1

HCID 6228Dimethylformamide溶媒

反応条件

温度
4 °C

実験手順

To N-TeocT4 (0.017 g, 17 μmol) in 1 mL dry DMF was added dicyclohexylcarbodiimide (0.004 g, 18 μmol). After stirring for 30 minutes N-dimethyl-4-aminopyridine (0.004 g, 36 μmol) and Gly18 (0.017 g, 17 μmol) were added and the reaction stirred overnight. The cloudy solution was poured into 20 mL H2O and extracted twice with 10 mL CH2Cl2. The aqueous component was acidified to pH 3 with 1 N HCl and chilled to 4° C. The material was isolated by centrifugation and the pellet washed 3 times with 8 mL H2O. The pellet was dried in vacuum to yield dicyclohexylurea and N-TeocT4-Gly18. (SEQ ID NO: 6)

後処理

  1. additionGly18 (0.017 g, 17 μmol) were added
  2. extractionextracted twice with 10 mL CH2Cl2
  3. customThe material was isolated by centrifugation
  4. washthe pellet washed 3 times with 8 mL H2O
  5. customThe pellet was dried in vacuum

出典と根拠

ORDレコード
ord-d889715e4eb84c7d91566f2edec9a1ef
出典データセット
uspto-grants-2008_09
特許番号
US07427600B2

関連ノート

読み込み中…