7-Chloro-3-methyl-1-phenyl-1,2,4,5-tetrahydro-3-benzazepin-8-ol → Dopamine

HRID 327593

反応式

HRID 327593 の反応式
C1C2(N(C3C=CC=CC=3)CNC2=[O:8])CCN(CCCC(C2C=CC(F)=CC=2)=O)C1.CC([C@@]1(O)C[C@@H]2N(C[C@@H]3C4C2=CC=CC=4CCC2C=CC=CC3=2)CC1)(C)C.C[N:58]1[CH2:68][CH:67](C2C=CC=CC=2)[C:66]2[C:61](=[CH:62][C:63](Cl)=[C:64]([OH:75])[CH:65]=2)CC1.CN1C[C@H](C2C=CC=CC=2)C2C=C(O)C(Cl)=CC=2CC1>>[NH2:58][CH2:68][CH2:67][C:66]1[CH:61]=[CH:62][C:63]([OH:8])=[C:64]([OH:75])[CH:65]=1

参加物

5

反応物 4

HCID 50187-Chloro-3-methyl-1-phenyl-1,2,4,5-tetrahydro-3-benzazepin-8-olHCID 5265SpiperoneHCID 37459(+)-ButaclamolHCID 3036864R-(+)-8-chloro-2,3,4,5-tetrahydro-3-methyl-5-phenyl-1H-3-benzazepine-7-ol

生成物 1

HCID 681Dopamine

実験手順

The following tritiated drugs were used as radioligands for each of the receptors tested: [3H]-Spiperone 21-24 Ci/mmol for D2 receptors and [3H]-SCH23390 75-85 Ci/mmol for D1 receptors. The radioligands were incubated with various concentrations of competing drug and the appropriate membrane source for periods of time as follows: 75 minutes at room temperature for D2 receptors or 15 minutes at 37° C. for D1 receptors. Specific binding was defined using 1 μM butaclamol (D2) or 1 μM SCH23390 (D1). In addition, the D2 assays contained 30 nM ketaserin in order to block the binding of 3H-spiperone to 5HT2 receptors.

後処理

  1. concentrationThe radioligands were incubated with various concentrations
  2. waitthe appropriate membrane source for periods of time as follows
  3. custom75 minutes
  4. customat room temperature
  5. customfor D2 receptors or 15 minutes
  6. customat 37° C.

出典と根拠

ORDレコード
ord-5ce47448ae38482fa84af9f96e6a1620
出典データセット
uspto-grants-1993_12
特許番号
US05274003

関連ノート

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