化工社化工社 化学情報印刷レポート · 研究参考用
L-Glutamine → Sodium Bicarbonate
関与物質
| 役割 | 名称 | HCID | 仕込み量 |
|---|---|---|---|
| 反応物 | L-Glutamine | HCID 5961 | — |
| 反応物 | Gentamicin C1 | HCID 72395 | — |
| 反応物 | Npc478664 | HCID 16051958 | — |
| 反応物 | Penicillin G Sodium | HCID 23668834 | — |
| 生成物 | 4-(2-Hydroxyethyl)-1-piperazineethanesulfonic acid | HCID 23831 | — |
| 生成物 | Sodium Bicarbonate | HCID 516892 | 0.075% |
反応手順
Human MCF-7 breast carcinoma cells were maintained in RPMI 1640 medium containing 100 units/mL penicillin G sodium, 100 μg/mL streptomycin sulfate, and 25 μg/mL gentamicin. The medium was supplemented with 10% heat-inactivated fetal bovine serum and 2 mM glutamine. Additional buffering was provided with 10 mM HEPES and 0.075% sodium bicarbonate. The tumor cells were cultured in tissue culture flasks in a humidified incubator at 37° C. in an atmosphere of 95% air and 5% CO2.
情報源と証拠
| DOI | 10.6084/m9.figshare.5104873.v1 |
|---|---|
| 特許番号 | US08877715B2 |
| 出典データセット | uspto-grants-2014_11 |
| ORD ID | ord-0cfa3d0c62b9470ea603501e1e2e5dc9 |
| 出典メモ | uspto-grants-2014_11 |