L-Glutamine → Sodium Bicarbonate

HRID 330755

反応式

HRID 330755 の反応式
CC1(C)S[C@@H:5]2[C@H:7]([NH:10][C:11]([CH2:13]C3C=CC=CC=3)=O)[C:8](=[O:9])[N:4]2[C@H:3]1[C:20]([O-:22])=O.[Na+:24].C[C@@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)[C@H](N=C(N)N)[C@@H](O)[C@@H]2N=C(N)N)[C@H]([O:49][C@@H:50]2[O:55][C@@H](CO)[C@H](O)[C@@H](O)[C@@H]2NC)[C@@]1(O)C=O.[OH:65][S:66]([OH:69])(=O)=[O:67].[CH3:70][C@@H](NC)[C@H]1O[C@H](O[C@H]2[C@H](O)[C@@H](O[C@H]3OC[C@@](O)(C)[C@H](NC)[C@H]3O)[C@H](N)C[C@@H]2N)[C@H](N)CC1.N[C@H](C(O)=O)CCC(=O)N>>[CH2:5]1[N:4]([CH2:3][CH2:20][OH:22])[CH2:8][CH2:7][N:10]([CH2:11][CH2:13][S:66]([OH:69])(=[O:67])=[O:65])[CH2:70]1.[C:50](=[O:55])([OH:9])[O-:49].[Na+:24]

参加物

6

反応物 4

HCID 5961L-GlutamineHCID 72395Gentamicin C1HCID 16051958Npc478664HCID 23668834Penicillin G Sodium

生成物 2

HCID 238314-(2-Hydroxyethyl)-1-piperazineethanesulfonic acidHCID 516892Sodium Bicarbonate収率 0.075%

実験手順

Human MCF-7 breast carcinoma cells were maintained in RPMI 1640 medium containing 100 units/mL penicillin G sodium, 100 μg/mL streptomycin sulfate, and 25 μg/mL gentamicin. The medium was supplemented with 10% heat-inactivated fetal bovine serum and 2 mM glutamine. Additional buffering was provided with 10 mM HEPES and 0.075% sodium bicarbonate. The tumor cells were cultured in tissue culture flasks in a humidified incubator at 37° C. in an atmosphere of 95% air and 5% CO2.

後処理

  1. temperatureHuman MCF-7 breast carcinoma cells were maintained in RPMI 1640 medium

出典と根拠

ORDレコード
ord-0cfa3d0c62b9470ea603501e1e2e5dc9
出典データセット
uspto-grants-2014_11
特許番号
US08877715B2

関連ノート

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