Sucrose → Spectinomycin

HRID 336029

反応式

HRID 336029 の反応式
C(O)[C@H]1O[C@H](O[C@]2(CO)O[C@H](CO)[C@@H](O)[C@@H]2O)[C@H](O)[C@@H](O)[C@@H]1O.C1(CC(O)=O)C2C(=CC=CC=2)C=CC=1.C(NC1N=C2C(NC=N2)=CN=1)C1C=CC=CC=1.[CH3:55][C@H:56]1[O:62][C@H:61]2[O:63][C@H:64]3[C@H:69]([O:70][C@@:60]2([OH:77])[C:58](=[O:59])[CH2:57]1)[C@@H:68]([NH:71][CH3:72])[C@@H:67]([OH:73])[C@@H:66]([NH:74][CH3:75])[C@@H:65]3[OH:76].Cl.Cl>>[CH3:55][C@H:56]1[O:62][C@H:61]2[O:63][C@H:64]3[C@H:69]([O:70][C@@:60]2([OH:77])[C:58](=[O:59])[CH2:57]1)[C@@H:68]([NH:71][CH3:72])[C@@H:67]([OH:73])[C@@H:66]([NH:74][CH3:75])[C@@H:65]3[OH:76]

参加物

5

反応物 4

HCID 5988SucroseHCID 68621-Naphthaleneacetic AcidHCID 64740Spectinomycin DihydrochlorideHCID 12922494N-Benzyl-7H-purin-2-amine

生成物 1

HCID 15541Spectinomycin

実験手順

When calli are about 0.5 mm in size they are transferred to callus growth medium SH2 (Schenk & Hildebrandt, Can. J. Bot. 50:199-204 (1972)) with 30 g/l saccharose, 5 g/l agarose, 0.1 mg/l 1-naphtalene acetic acid (NAA) and 0.1 mg/l benzylaminopurin (BAP), and the selecting agent spectinomycin dihydrochloride at concentrations of 0-1000 mg/l. It was found that the optimal concentration of selection is 500 mg/l. The non-resistant calli appear as white calli. They also grow slower as compared to the control calli. The culture conditions are as described above for the above protoplast calli.

後処理

  1. concentrationIt was found that the optimal concentration of selection

出典と根拠

ORDレコード
ord-252de01952f54d03bdc6ddb409cdb7fa
出典データセット
uspto-grants-2014_11
特許番号
US08884099B2

関連ノート

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