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Pyridoxal 5'-phosphate → Indole
関与物質
| 役割 | 名称 | HCID | 仕込み量 |
|---|---|---|---|
| 反応物 | Pyridoxal 5'-phosphate | HCID 1051 | — |
| 反応物 | L-Tryptophan | HCID 6305 | — |
| 反応物 | Trichloroacetic Acid | HCID 6421 | — |
| 反応物 | Monopotassium phosphate dipotassium phosphate | HCID 21889592 | — |
| 生成物 | Indole | HCID 798 | — |
反応手順
Measured in accordance with the method of Yamada et al. as follows. In 4.0ml of a reaction mixture containing 10 micromoles of L-tryptophan, 0.4 micromole of pyridoxalphosphate (PLP)), 200 micromoles of K2HPO4 -KH2PO4 (pH 8.0), the enzyme is incubated at 65° C. for 10 minutes. Then, 1.0 ml of a 30% aqueous solution of trichloroacetic acid (TCA) is added to stop the reaction. Indole formed in the reaction system is quantitatively determined by the method of E. McEvoy-Bowe [The ANALYST, vol. 88, pages 893-894 (1963)]. The activity is expressed in units in which 1 U is the amount in micromoles of indole formed during 1 minute.
情報源と証拠
| DOI | 10.6084/m9.figshare.5104873.v1 |
|---|---|
| 特許番号 | US04761374 |
| 出典データセット | uspto-grants-1988_08 |
| ORD ID | ord-206ec9e44fcb496288f80b4c23c1714c |
| 出典メモ | uspto-grants-1988_08 |