Phosphate → 222555-05-7

HRID 351508ノート

反応式

HRID 351508 の反応式
[CH2:1]([O:3][CH:4]([CH2:10][C:11]1[CH:16]=[CH:15][C:14]([OH:17])=[CH:13][CH:12]=1)[C:5]([O:7][CH2:8][CH3:9])=[O:6])[CH3:2].P([O-])([O-])([O-])=O>CC(C)=O>[CH2:1]([O:3][C@H:4]([CH2:10][C:11]1[CH:12]=[CH:13][C:14]([OH:17])=[CH:15][CH:16]=1)[C:5]([O:7][CH2:8][CH3:9])=[O:6])[CH3:2]

参加物

4

反応物 2

HCID 1061PhosphateHCID 11791243Ethyl 2-ethoxy-3-(4-hydroxyphenyl)propanoate

生成物 1

HCID 10220356222555-05-7収率 82.7%

試薬・触媒・溶媒 1

HCID 180Acetone溶媒

実験手順

Enantiomerically enriched (ee Renantiomer=60%) ethyl 2-ethoxy-3-(4-hydroxyphenyl)propanoate (5.2 g) was dissolved in 30 ml acetone and added to an aqueous 0.1 M phosphate buffer pH 7 (1.0 l). Protease 2A from Aspergillus oryzae (Fluka No: 82463; 0.51 units/mg) (13 g) was added and the mixture was stirred for 3 days at room temperature. The reaction mixture was extracted 4× with 200 ml TBME. After drying of the combined organic phases over Na2SO4and evaporation of the TBME, 4.3 g of ethyl (2R)-2-Ethoxy-3-(4-hydroxyphenyl)propanoate was obtained as an oil (CCE method 2: ee=100%).

後処理

  1. addition0.51 units/mg) (13 g) was added
  2. extractionThe reaction mixture was extracted 4× with 200 ml TBME
  3. dry with materialAfter drying of the combined organic phases over Na2SO4and evaporation of the TBME

出典と根拠

ORDレコード
ord-7b800efa21594ed087afb6a664cb8bf1
出典データセット
uspto-grants-2004_06
特許番号
US06743608B2

反応ノート

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