Ethoxyethyl (2rs) 2-ethoxy-3-(4-hydroxyphenyl)propanoate → HCID 86587059、化合物
反応式
[CH2:1]([O:3][CH:4]([CH2:13][C:14]1[CH:19]=[CH:18][C:17]([OH:20])=[CH:16][CH:15]=1)[C:5]([O:7][CH2:8][CH2:9][O:10][CH2:11][CH3:12])=[O:6])[CH3:2]>P([O-])([O-])([O-])=O>[CH2:1]([O:3][C@@H:4]([CH2:13][C:14]1[CH:15]=[CH:16][C:17]([OH:20])=[CH:18][CH:19]=1)[C:5]([OH:7])=[O:6])[CH3:2].[CH2:1]([O:3][C@H:4]([CH2:13][C:14]1[CH:19]=[CH:18][C:17]([OH:20])=[CH:16][CH:15]=1)[C:5]([O:7][CH2:8][CH2:9][O:10][CH2:11][CH3:12])=[O:6])[CH3:2]参加物
3
実験手順
To ethoxyethyl (2RS) (+/−) 2-ethoxy-3-(4-hydroxyphenyl)-propanoate (0.25 ml of a solution containing 2 mg/ml in phosphate buffer 0.1M pH 7) was added the enzyme (amount indicated below) diluted in phosphate buffer 0.1M pH 7 (buffer volume such as total reaction mixture volume was 0.5 ml). The reaction mixture was shaken at room temperature and analysed at different times. The reaction mixture was analysed by the gradient HPLC method 1, and the chiral CE method 2.
後処理
- additionwas added the enzyme (amount indicated below)