Nitrophenylgalactosides → Indole

HRID 35800

反応式

HRID 35800 の反応式
[CH:1]1[CH:6]=[C:5]([N+:7]([O-])=O)[C:4](O[C@@H]2O[C@H](CO)[C@H](O)[C@H](O)[C@H]2O)=[CH:3][CH:2]=1.O[CH:23]1O[C@H](CO)[C@@H](O[C@@H]2O[C@H](CO)[C@H](O)[C@H](O)[C@H]2O)[C@H](O)[C@H:24]1O>>[NH:7]1[C:5]2[C:4](=[CH:3][CH:2]=[CH:1][CH:6]=2)[CH:24]=[CH:23]1

参加物

3

反応物 2

HCID 96647Nitrophenylgalactosides2 回HCID 440995(+)-Lactose

生成物 1

HCID 798Indole

実験手順

Principle of Step 1: ONPG substituting for lactose is metabolized with β-galactosidase. At 44.5° C. E. coli grow and utilize ONPG and produce indole. β-galactoside turns the medium yellow. In the yellow colored medium, both fecal coliforms and E. coli are present. In samples which do not have a yellow color, there are no fecal coliforms and E. coli.

後処理

  1. customAt 44.5° C

出典と根拠

ORDレコード
ord-132faaf6deb4480d81ce4d9a519ddd7a
出典データセット
uspto-grants-1995_05
特許番号
US05411867

関連ノート

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