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Calcium Acetate → 4-Nitroaniline
関与物質
| 役割 | 名称 | HCID | 仕込み量 |
|---|---|---|---|
| 反応物 | Calcium Acetate | HCID 6116 | — |
| 反応物 | Chromozym TH hydrochloride | HCID 178427468 | — |
| 生成物 | 4-Nitroaniline | HCID 7475 | — |
| 生成物 | HCID 178427469 | HCID 178427469 | — |
反応手順
After precisely 4 minutes, 150 μl. of starting reagent, consisting of 1.1 mmole/litre Chromozym® TH (Tos-Gly-ProArg-pNA) and 100 mmole/litre calcium acetate, are added thereto and at 405 nm there is determined in a photometer the time until a definite amount of substrate is reacted by newly formed thrombin to give Tos-Gly-Pro-Arg and p-nitroaniline (pNA). The amount of reacted substrate, which is measured, is defined by a threshold extinction value (for example ΔE=0.2).
情報源と証拠
| DOI | 10.6084/m9.figshare.5104873.v1 |
|---|---|
| 特許番号 | US05001069 |
| 出典データセット | uspto-grants-1991_03 |
| ORD ID | ord-6e2ad97def424f37a65c9f83ecd56811 |
| 出典メモ | uspto-grants-1991_03 |