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Nitrous Acid → 3-Methyl-2-benzothiazolone hydrazone
関与物質
| 役割 | 名称 | HCID | 仕込み量 |
|---|---|---|---|
| 反応物 | Nitrous Acid | HCID 24529 | — |
| 反応物 | 3-Methyl-2-benzothiazolinone hydrazone hydrochloride | HCID 78041 | — |
| 反応物 | D-Glucosamine | HCID 439213 | — |
| 生成物 | 3-Methyl-2-benzothiazolone hydrazone | HCID 5782694 | — |
反応手順
Nitrous acid oxidation followed by reaction with 3-methyl-2-benzothiazolone hydrazone hydrochloride (MBTH) and then FeCl3 was used to measure the number of free amino groups (equivalent to the number of glucosamines) in the glycolchitosan and derived products (Davies et al., Meth. Enzymol. 161:442-446 (1988)). The assay was standardized with glucosamine on a weight basis. Also the MBTH/Fe3+ stage of this procedure was used to measure the number of aldehyde groups, similarly relying on standardization with glucosamine treated with nitrous acid.
情報源と証拠
| DOI | 10.6084/m9.figshare.5104873.v1 |
|---|---|
| 特許番号 | US05412083 |
| 出典データセット | uspto-grants-1995_05 |
| ORD ID | ord-4f9855f6bfe048f0b7fd8b36d40e0492 |
| 出典メモ | uspto-grants-1995_05 |