化工社化工社 化学情報印刷レポート · 研究参考用
Ethylenediaminetetraacetic Acid → Glutathione diethyl ester
関与物質
| 役割 | 名称 | HCID | 仕込み量 |
|---|---|---|---|
| 反応物 | Ethylenediaminetetraacetic Acid | HCID 6049 | — |
| 反応物 | 5,5'-Dithiobis(2-nitrobenzoic Acid) | HCID 6254 | — |
| 反応物 | Glutathione | HCID 124886 | — |
| 溶媒 | Acetic Acid | HCID 176 | — |
| 生成物 | Glutathione diethyl ester | HCID 175702 | — |
反応手順
Analysis of Erythrocyte Thiol by DTNB. Cell supernatant (40 μl) was added to 1 ml of 6 mM DTNB (in 143 mM phosphate buffer, pH 7.5, containing 6.3 mM EDTA). The change in absorbance at 412 nm after 1 min was converted to a value for total thiol using a GSH standard curve. The GSH standard were prepared in a 1:2:2 mixture of PBS: 0.435M acetic acid: 10% SSA (containing 2 mM EDTA) ("RBC Mix").
情報源と証拠
| DOI | 10.6084/m9.figshare.5104873.v1 |
|---|---|
| 特許番号 | US05869456 |
| 出典データセット | uspto-grants-1999_02 |
| ORD ID | ord-f1378040555142079caca127d22fad11 |
| 出典メモ | uspto-grants-1999_02 |