反応式
C(O)[C:2](N)([CH2:5][OH:6])[CH2:3][OH:4].[OH:9][CH2:10][CH:11]([CH2:13][OH:14])[OH:12].[SH:15][CH:16](O)[CH3:17].[CH3:19]CCCCCCCCCCCOS([O-])(=O)=O.[Na+]>>[CH3:19][CH:16]([S:15][C@@H:10]1[O:9][C@H:2]([CH2:3][OH:4])[C@H:5]([OH:6])[C@H:13]([OH:14])[C@H:11]1[OH:12])[CH3:17]参加物
5
実験手順
Bacterial cells were harvested by centrifugation and resuspended in SDS sample buffer (0.0625M Tris, pH 6.8, 10% glycerol, 5% mercaptoethanol, 2.3% SDS). The resuspended pellet was boiled for 5 min. and then cleared of insoluble cellular debris by centrifugation. The supernatants obtained from IPTG-induced cultures of GE3-2, GE9-2, GE15-1, GE17-2, GE4-8, EXP3-7, GE1-N and GE57 were analyzed by SDS-polyacrylamide gel electrophoresis (PAGE) together with uninduced lysates. The proteins from these gels were then transferred to nitrocellulose filters (i.e., by Western blotting).
出典と根拠
- ORDレコード
- ord-b4112dba39db4f869c590e5c996c75c1
- 出典データセット
- uspto-grants-1999_02
- 特許番号
- US05874563
関連ノート
読み込み中…