反応式
[3H]C1C=C2OCOC2=CC=1OC[C@H:10]1[C@H:15]([C:16]2[CH:17]=[CH:18][C:19](F)=[CH:20][CH:21]=2)CC[NH:12][CH2:11]1.[Na+].[Cl-].[C-:28]#[N:29].[K+].CN(CCCC1(C2C=CC(F)=CC=2)[O:45]CC2C=C(C#N)C=CC1=2)C>>[CH:19]1[C:18]([OH:45])=[CH:17][C:16]2[C:15]([CH2:10][CH2:11][NH2:12])=[CH:28][NH:29][C:21]=2[CH:20]=1参加物
5
実験手順
The affinity was determined by competition experiments using [3H]-paroxetine. The membranes are prepared from the frontal cortex of the rat and are incubated in triplicate with 0.25 nM [3H]-paroxetine and the compound being tested in a final volume of 0.4 ml, for 2 hours at 25° C. The incubation buffer contains 50 mM TRIS-HCl (pH 7.4), 120 mM NaCl and 5 mM KCN. The non-specific binding is determined using 10 μM citalopram.
後処理
- customThe membranes are prepared from the frontal cortex of the rat
出典と根拠
- ORDレコード
- ord-263e313e170c4a58b266a05ef0b905c2
- 出典データセット
- uspto-grants-2000_10
- 特許番号
- US06127396
関連ノート
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