Adenosine → 2'-Deoxy-2'-fluoroguanosine

HRID 37068

反応式

HRID 37068 の反応式
[NH2:1][C:2]1[N:10]=[C:9]2[C:5]([N:6]=[CH:7][N:8]2[C@@H:11]2[O:16][C@H:15]([CH2:17][OH:18])[C@@H:13]([OH:14])[C@H:12]2[F:19])=[C:4](N)[N:3]=1.[C@@H]1(N2C3N=CN=C(N)C=3N=C2)O[C@H](CO)[C@@H](O)[C@H]1[OH:23]>O>[F:19][C@@H:12]1[C@H:13]([OH:14])[C@@H:15]([CH2:17][OH:18])[O:16][C@H:11]1[N:8]1[CH:7]=[N:6][C:5]2[C:4](=[O:23])[NH:3][C:2]([NH2:1])=[N:10][C:9]1=2

参加物

4

反応物 2

HCID 60961AdenosineHCID 99711012-Amino-2'-deoxy-2'-fluoroadenosine

生成物 1

HCID 1354155652'-Deoxy-2'-fluoroguanosine

試薬・触媒・溶媒 1

HCID 962Water溶媒

反応条件

温度
4 °C

実験手順

2,6-Diamino-9-(2-deoxy-2-fluoro-β-D-ribofuranosyl)-9H-purine (0.20 g, 0.64 mmole), prepared as in Example 6, was dissolved in 15 ml of water. Calf intestinal adenosine deaminase (4 I.U., Boehringer Mannheim) was added and the solution incubated at 37° for 4 days. The solution was cooled to 4° C. After 3 hrs, the suspension was filtered to remove the first batch of product crystals. The volume of the filtrate was reduced under vacuum and the suspension cooled to 4° C. The suspension was filtered to remove the second batch of product crystals. The batches of product crystals were combined, suspended in water and lyophilized to obtain title compound that analyzed as a 1.3 hydrate.

後処理

  1. waitAfter 3 hrs
  2. filtrationthe suspension was filtered
  3. customto remove the first batch of product crystals
  4. temperaturethe suspension cooled to 4° C
  5. filtrationThe suspension was filtered
  6. customto remove the second batch of product crystals

出典と根拠

ORDレコード
ord-7aa613c29b004721978c946d018d6d83
出典データセット
uspto-grants-1995_05
特許番号
US05420115

関連ノート

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