Adenosine → 2'-Deoxy-2'-fluoroinosine

HRID 37079

反応式

HRID 37079 の反応式
N[C:2]1[N:10]=[CH:9][N:8]=[C:7]2[C:3]=1[N:4]=[CH:5][N:6]2[C@@H:11]1[O:16][C@H:15]([CH2:17][OH:18])[C@@H:13]([OH:14])[C@H:12]1[F:19].[C@@H]1(N2C3N=CN=C(N)C=3N=C2)O[C@H](CO)[C@@H](O)[C@H]1[OH:22]>O>[F:19][C@@H:12]1[C@H:13]([OH:14])[C@@H:15]([CH2:17][OH:18])[O:16][C@H:11]1[N:6]1[CH:5]=[N:4][C:3]2[C:2](=[O:22])[NH:10][CH:9]=[N:8][C:7]1=2

参加物

4

反応物 2

HCID 60961AdenosineHCID 1002532'-Fluoro-2'-deoxyadenosine

生成物 1

HCID 1354351722'-Deoxy-2'-fluoroinosine

試薬・触媒・溶媒 1

HCID 962Water溶媒

実験手順

6-Amino-9-(2-deoxy-2-fluoro-β-D-ribofuranosyl)-9H-purine (0.29 g, 1.0 mmole), prepared as in Example 8, was dissolved in 100 ml of water. Calf intestinal adenosine deaminase (4 I.U., Boehringer Mannheim) was added and the solution incubated at 37° C. for 24 hrs. The solvent was removed under vacuum. The residue was dissolved in water/n-propanol (7/3) and chromatographed on a 5×90 cm column of Sephadex G-10 (Pharmacia LKB) with water/n-propanol (7/3) as the solvent. Product-containing fractions were combined and the solvent removed under vacuum. The residue was dissolved in water and lyophilized to obtain title compound that analyzed as a hydrate.

後処理

  1. customThe solvent was removed under vacuum
  2. dissolutionThe residue was dissolved in water/n-propanol (7/3)
  3. customchromatographed on a 5×90 cm column of Sephadex
  4. customthe solvent removed under vacuum
  5. dissolutionThe residue was dissolved in water

出典と根拠

ORDレコード
ord-24624ba4ad42404e88c2b51eb4f0dcd5
出典データセット
uspto-grants-1995_05
特許番号
US05420115

関連ノート

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