Acetate → HCID 70619535、化合物

HRID 372778ノート

反応式

HRID 372778 の反応式
Cl.[NH2:2][C@H:3]([C:12]([NH:14][CH2:15][C:16]([NH:18][CH2:19][C:20]([NH:22][C@H:23]([C:34]([NH:36][C@H:37]([C:42]([OH:44])=[O:43])[CH2:38][CH2:39][S:40][CH3:41])=[O:35])[CH2:24][C:25]1[C:33]2[C:28](=[CH:29][CH:30]=[CH:31][CH:32]=2)[NH:27][CH:26]=1)=[O:21])=[O:17])=[O:13])[CH2:4][C:5]1[CH:10]=[CH:9][C:8]([OH:11])=[CH:7][CH:6]=1.C([O-])(=O)C>C(O)(=O)C>[C:42]([OH:44])(=[O:43])[CH3:37].[NH2:2][C@H:3]([C:12]([NH:14][CH2:15][C:16]([NH:18][CH2:19][C:20]([NH:22][C@H:23]([C:34]([NH:36][C@H:37]([C:42]([OH:44])=[O:43])[CH2:38][CH2:39][S:40][CH3:41])=[O:35])[CH2:24][C:25]1[C:33]2[C:28](=[CH:29][CH:30]=[CH:31][CH:32]=2)[NH:27][CH:26]=1)=[O:21])=[O:17])=[O:13])[CH2:4][C:5]1[CH:10]=[CH:9][C:8]([OH:11])=[CH:7][CH:6]=1

参加物

4

反応物 2

HCID 175AcetateHCID 70620434HCID 70620434、化合物

生成物 1

HCID 70619535HCID 70619535、化合物

試薬・触媒・溶媒 1

HCID 176Acetic Acid溶媒

実験手順

17.3 Parts L-tyrosylglycylglycyl-L-tryptophyl-L-methionine hydrochloride is dissolved in 250 parts by volume of 20% acetic acid and passed slowly through an IR-45 ion exchange column in the acetate form. The column is washed with 20% acetic acid until no more peptide is eluted. Fractions containing the product are combined and the solvent removed by stripping under reduced pressure at room temperature. The residual glass is dissolved in 75 parts water and lyophilized to give L-tyrosylglycylglycyl-L-tryptophyl-L-methionine acetic acid salt. This compound is represented by the following formula

後処理

  1. washThe column is washed with 20% acetic acid until no more peptide
  2. washis eluted
  3. additionFractions containing the product
  4. customthe solvent removed
  5. customby stripping under reduced pressure at room temperature
  6. dissolutionThe residual glass is dissolved in 75 parts water

出典と根拠

ORDレコード
ord-b6539617919848d1a54a7a3195315bce
出典データセット
uspto-grants-1978_05
特許番号
US04092304

反応ノート

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