Phosphate → HCID 178508926、化合物

HRID 373662

反応式

HRID 373662 の反応式
[CH:1]1[N:5]([C@@H:6]2[O:9][C@H:8]([CH2:10][OH:11])[C@H:7]2[CH2:12][OH:13])[C:4]2[N:14]=[C:15]([NH2:19])[N:16]=[C:17](N)[C:3]=2[N:2]=1.P([O-])([O-])([O-])=[O:21]>[C@@H]1(N2C3N=CN=C(N)C=3N=C2)O[C@H](COP(O)(O)=O)[C@@H](O)[C@H]1O>[CH:1]1[N:5]([C@@H:6]2[O:9][C@H:8]([CH2:10][OH:11])[C@H:7]2[CH2:12][OH:13])[C:4]2[NH:14][C:15]([NH2:19])=[N:16][C:17](=[O:21])[C:3]=2[N:2]=1

参加物

4

反応物 2

HCID 1061PhosphateHCID 4515592-Aminooxetanocin A

生成物 1

HCID 178508926HCID 178508926、化合物

試薬・触媒・溶媒 1

HCID 6083Adenosine Monophosphate溶媒

反応条件

温度
37 °C

実験手順

After 2.0 g of 2-amino-OXT-A was dissolved in 300 ml of 1/10M phosphate buffer (pH 6.5), 200 units of 5'-adenylic acid deaminase (manufactured by Sigma Co., A-1907) were added to the solution followed by stirring at 37° C. for 50 hours. After the reaction solution was passed through a column packed with MCI® GEL CHP 20 (300 ml) and the reaction products were adsorbed onto it, the products were eluted by water. Fractions showing an Rf value of about 0.42 in silica gel TLC [developing solvent: n-butanol-acetic acid-water (4:1:2)] were collected and concentrated to dryness under reduced pressure to give 1.8 g of OXT-G as colorless crystals (yield, 90%).

後処理

  1. addition, A-1907) were added to the solution
  2. washthe products were eluted by water
  3. customwere collected
  4. concentrationconcentrated to dryness under reduced pressure
  5. customto give

出典と根拠

ORDレコード
ord-b18c4afd2c6c4adb8bfac8575dfa5d7b
出典データセット
uspto-grants-1992_11
特許番号
US05164500

関連ノート

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