Adenosine Monophosphate → HCID 178508926、化合物

HRID 373663

反応式

HRID 373663 の反応式
[CH:1]1[N:5]([C@@H:6]2[O:9][C@H:8]([CH2:10][OH:11])[C@H:7]2[CH2:12][OH:13])[C:4]2[N:14]=[C:15]([NH2:19])[N:16]=[C:17](N)[C:3]=2[N:2]=1.[C@@H]1(N2C3N=CN=C(N)C=3N=C2)O[C@H](COP(O)(O)=O)[C@@H](O)[C@H]1[OH:22]>P([O-])([O-])([O-])=O>[CH:1]1[N:5]([C@@H:6]2[O:9][C@H:8]([CH2:10][OH:11])[C@H:7]2[CH2:12][OH:13])[C:4]2[NH:14][C:15]([NH2:19])=[N:16][C:17](=[O:22])[C:3]=2[N:2]=1

参加物

4

反応物 2

HCID 6083Adenosine MonophosphateHCID 4515592-Aminooxetanocin A

生成物 1

HCID 178508926HCID 178508926、化合物

試薬・触媒・溶媒 1

HCID 1061Phosphate溶媒

反応条件

温度
37 °C

実験手順

After 2.0 g of 2-amino-OXT-A was dissolved in 300 ml of 1/10M phosphate buffer (pH 6.5), 8.0 g of 5'-adenylic acid deaminase (manufactured by Amano Pharmaceutical Co., Ltd., DEAMIZYME®) was added to the solution followed by stirring at 37° C. for 70 hours. After insoluble matters of the reaction solution were removed by filtration, the filtrate was passed through a column packed with 50 ml of activated charcoal powders (manufactured by Wako Pure Chemical Industry K.K., for chromatography). After washing with water, elution was performed with 50% aqueous methanol. By concentrating the desired fraction to dryness, crude OXT-G was obtained. The crude powders were dissolved in 50 ml of water and the OXT-G in the solution was adsorbed onto MCI® GEL CHP 20 (300 ml) followed by elution with water. The desired fraction was concentrated and evaporated to dryness under reduced pressure to give 1.5 g of OXT-G as colorless crystals (yield, 75%).

後処理

  1. additionwas added to the solution
  2. customAfter insoluble matters of the reaction solution were removed by filtration
  3. washAfter washing with water, elution
  4. concentrationBy concentrating the desired fraction to dryness

出典と根拠

ORDレコード
ord-152f04f6ff8c402b83461295500c4937
出典データセット
uspto-grants-1992_11
特許番号
US05164500

関連ノート

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