(2R,3S,4R,5R)-2,3,4,5,6-pentahydroxyhexanal → HCID 178418489、化合物

HRID 383222

反応式

HRID 383222 の反応式
[O:1]=[CH:2][C@@H:3]([C@H:5]([C@@H:7]([C@@H:9](CO)[OH:10])[OH:8])[OH:6])[OH:4].O=C[C@@H]([C@H]([C@@H](CO)O)O)O>C(O)C>[CH2:2]([OH:1])[C@@H:3]([C@H:5]([C@@H:7]([CH2:9][OH:10])[OH:8])[OH:6])[OH:4]

参加物

4

反応物 2

HCID 107526(2R,3S,4R,5R)-2,3,4,5,6-pentahydroxyhexanalHCID 644160aldehydo-D-Xylose

生成物 1

HCID 178418489HCID 178418489、化合物

試薬・触媒・溶媒 1

HCID 702Ethanol溶媒

実験手順

Strains Y-PsNative, Y-PsK270M and Y-PsK270R (cf example 4) were compared in anaerobic batch fermentation with 20 g l−1 glucose and 50 g l−1 xylose (FIG. 3). Table 6 summarizes xylose consumption, ethanol concentration and product yields after 117 hours of fermentation. The reference strain Y-PsNative consumed 30.4 g l−1 xylose and produced 16.7 g l−1 ethanol while Y-PsK270R consumed 46.1 g l−1 xylose and produced 25.3 g l−1 ethanol. Y-PsK270M consumed the least xylose (16.8 g l−1) and produced the lowest ethanol concentration (14.1 g l−1) of the three strains. The reference strain Y-PsNative produced an ethanol yield of 0.33 g ethanol g consumed sugars−1 and a xylitol yield of 0.26 g xylitol g consumed xylose−1. Both strains with mutated XR produced higher ethanol yields (0.38 g ethanol (g consumed sugars)−1) and significantly lower xylitol yields (0.09 g xylitol (g consumed xylose)−1) than the reference strain. In addition, Y-PsK270R grew and produced biomass anaerobically from xylose after glucose depletion (FIG. 4). Y-PsK270R had produced 3.4 g l−1 biomass after 117 hours of fermentation while both Y-PsNative and Y-PsK270M produced 2.1 g l−1 biomass.

後処理

  1. customxylose consumption, ethanol concentration and product
  2. customyields after 117 hours of fermentation
  3. customThe reference strain Y-PsNative consumed 30.4 g l−1 xylose
  4. customproduced 16.7 g l−1 ethanol
  5. customwhile Y-PsK270R consumed 46.1 g l−1 xylose
  6. customproduced 25.3 g l−1 ethanol
  7. customY-PsK270M consumed the least xylose (16.8 g l−1)
  8. customproduced
  9. concentrationthe lowest ethanol concentration (14.1 g l−1) of the three strains
  10. customThe reference strain Y-PsNative produced an ethanol yield of 0.33 g ethanol
  11. customg consumed sugars−1
  12. customa xylitol yield of 0.26 g xylitol g consumed xylose−1
  13. customBoth strains with mutated XR produced higher ethanol

出典と根拠

ORDレコード
ord-7dfc20e410c4461792a3ef6bd70e3bcb
出典データセット
uspto-grants-2013_02
特許番号
US08367393B2

関連ノート

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