関与物質
| 役割 | 名称 | HCID | 仕込み量 |
|---|
| 反応物 | Chloramphenicol | HCID 5959 | — |
| 反応物 | Ampicillin | HCID 6249 | — |
| 反応物 | 4-Hydroxybutanoate | HCID 3037032 | — |
| 反応物 | 3-Hydroxypropionate | HCID 5459847 | — |
| 生成物 | Methyl Lactate | HCID 11040 | — |
| 生成物 | Butanoic acid, 4-hydroxy-, methyl ester | HCID 70217 | — |
| 生成物 | Propanoic acid, 3-hydroxy-, methyl ester | HCID 80252 | — |
| 生成物 | 4-Hydroxybutyrate 3-hydroxypropionate lactate | HCID 86645087 | — |
反応手順
4-Hydroxybutyrate-3-hydroxypropionate-lactate terpolymer was prepared according to the method of example 2 except that the collected cells was anaerobically cultured for 3 days in MR medium further containing 2 g/L of 4-hydroxybutyrate (4-HB), 2 g/L of 3-hydroxypropionate (3-HP), 100 mg/L of ampicillin and 30 mg/L of chloramphenicol instead of MR medium further containing g/L of 4-HB, 100 mg/L of ampicillin and 30 mg/L of chloramphenicol. —As a result of the analysis, methyl-4-hydroxybutyrate, methyl-3-hydroxypropionate and methyl-lactate were detected in E. coli Top10/pPs619C1300-CPPCT/pMCSPtbBuk transformant, which meant that new 4-hydroxybutyrate-3-hydroxypropionate-lactate terpolymer [poly(4-hydroxybutyrate-co-3-hydroxypropionate-co-lactate)] was prepared by the recombinant E. Coli. 1H-NMR and GC-MSD results of the obtained 4-hydroxybutyrate-3-hydroxypropionate-lactate terpolymer were shown in FIGS. 6 and 7, respectively.
情報源と証拠
| DOI | 10.6084/m9.figshare.5104873.v1 |
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| 特許番号 | US08383379B2 |
|---|
| 出典データセット | uspto-grants-2013_02 |
|---|
| ORD ID | ord-2c4161ef867a4cf4a9cfaee7a1d65950 |
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| 出典メモ | uspto-grants-2013_02 |
|---|
