5-(1-Propynyl)-2'-deoxyuridine → 5-Propynyluracil

HRID 398798

反応式

HRID 398798 の反応式
[C:1]([C:4]1[C:5](=[O:19])[NH:6][C:7](=[O:18])[N:8]([CH:17]=1)[C@@H]1O[C@H](CO)[C@@H](O)C1)#[C:2][CH3:3]>P([O-])([O-])([O-])=O>[C:1]([C:4]1[C:5](=[O:19])[NH:6][C:7](=[O:18])[NH:8][CH:17]=1)#[C:2][CH3:3]

参加物

3

反応物 1

HCID 4802655-(1-Propynyl)-2'-deoxyuridine

生成物 1

HCID 1318135-Propynyluracil

試薬・触媒・溶媒 1

HCID 1061Phosphate溶媒

実験手順

To a stirred solution of 2'-deoxy-5-propynyluridine (European Patent Specification No. 272065) (20g, 75 mmol) in aqueous phosphate buffer at pH 6.84 (1250 mL) was added purified E.coli thymidine phosphorylase (10,000 units) (T.A. Krenitsky et al, Biochemistry, 20, 3615, 1981; U.S. Patent Specification No. 4,381,344) and alkaline phosphatase (10,000 units) (Sigma type VII-S from bovine intestinal mucosa) and the whole mixture was incubated at 37° C. for 24 hours. The resulting white precipitate was filtered, washed with water (3 ×100 mL), ethanol (2 ×100 mL), ether (2 ×100 mL) and dried in vacuo over phosphorus pentoxide to give the title compound.

後処理

  1. custompurified E.coli thymidine phosphorylase (10,000 units) (T.A. Krenitsky et al, Biochemistry, 20, 3615, 1981; U.S. Patent Specification No. 4,381,344) and alkaline phosphatase (10,000 units) (Sigma type VII-S from bovine intestinal mucosa)
  2. filtrationThe resulting white precipitate was filtered
  3. washwashed with water (3 ×100 mL), ethanol (2 ×100 mL), ether (2 ×100 mL)
  4. dry with materialdried in vacuo over phosphorus pentoxide

出典と根拠

ORDレコード
ord-aa3ab387bbd140b7b484e3b00f1c21fc
出典データセット
uspto-grants-1998_10
特許番号
US05817664

関連ノート

読み込み中…