化工社化工社 化学情報印刷レポート · 研究参考用
Phosphoenolpyruvate → (2R,3S,4R,5R)-2,3,4,5,6-pentahydroxyhexanal
関与物質
| 役割 | 名称 | HCID | 仕込み量 |
|---|---|---|---|
| 反応物 | Phosphoenolpyruvate | HCID 1005 | — |
| 反応物 | Potassium Chloride | HCID 4873 | — |
| 反応物 | 5'-Atp | HCID 5957 | — |
| 反応物 | Pyruvate | HCID 107735 | — |
| 反応物 | Magnesium Chloride | HCID 5360315 | — |
| 反応物 | L-Ascorbic Acid | HCID 54670067 | — |
| 溶媒 | 4-Morpholineethanesulfonic acid | HCID 78165 | — |
| 生成物 | (2R,3S,4R,5R)-2,3,4,5,6-pentahydroxyhexanal | HCID 107526 | — |
反応手順
In 10.0 mM 2-morpholinoethanesulfonic acid (MES) buffer, 17.6 mM MgCl2, 17.6 mM KCl, 175.7 mM phosphoenolpyruvic acid (PEP), 17.6 mM ATP, 123 U/mL pyruvate kinase (PK), 97 U/mL hexokinase, and 20 U/mL ascorbic acid oxidase were dissolved in this composition, and the pH was adjusted to 7.0 to obtain a glucose converting reagent.
情報源と証拠
| DOI | 10.6084/m9.figshare.5104873.v1 |
|---|---|
| 特許番号 | US08753832B2 |
| 出典データセット | uspto-grants-2014_06 |
| ORD ID | ord-e8dc61f2a583462c83b2582574c4ed4b |
| 出典メモ | uspto-grants-2014_06 |