Iodine → Beta-D-Glucose 6-phosphate

HRID 441477ノート

反応式

HRID 441477 の反応式
C(=O)(O)[O-].[Na+].[CH2:6]([O:17][P:18]([OH:21])([OH:20])=[O:19])[CH:7]([OH:16])[CH:8]([OH:15])[CH:9]([OH:14])[CH:10]([OH:13])[CH:11]=[O:12].[Ba].C1N=C(N)C2N=CN([C@@H]3O[C@H](COP(OP(OC[C@H]4O[C@@H](N5C=C(C(N)=O)CC=C5)[C@H](O)[C@@H]4O)(O)=O)(O)=O)[C@@H](O)[C@H]3O)C=2N=1.II>CN(C=O)C>[CH2:6]([O:17][P:18]([OH:21])([OH:20])=[O:19])[C@H:7]1[O:16][C@@H:11]([OH:12])[C@H:10]([OH:13])[C@@H:9]([OH:14])[C@@H:8]1[OH:15]

参加物

6

反応物 4

HCID 807IodineHCID 439153NadhHCID 516892Sodium BicarbonateHCID 16219436Barium--6-O-phosphonohexose (1/1)

生成物 1

HCID 439427Beta-D-Glucose 6-phosphate

試薬・触媒・溶媒 1

HCID 6228Dimethylformamide溶媒3 回

実験手順

Following the procedure described above, 10mg of the T4 methyl ester diglycolate in 0.52ml dry DMF was reacted with 1.3mg NHS and 2.3mg ECDI. To 8.7mg G-6-PDH (0.08μ mole) in 1.65ml 1mg/ml sodium bicarbonate was added 5mg G-6-P and 12.5mg NADH, followed by 0.335ml DMF, followed by 9.5μmole ester prepared above in 0.215ml DMF. In 4 hours, a 47% loss in activity was observed. The hapten number was found to be 12 by UV and 13.2 by iodine analysis.

後処理

  1. customwas reacted with 1.3mg NHS and 2.3mg ECDI

出典と根拠

ORDレコード
ord-c08a2a382b3b4e68af4b574d4ed770bf
出典データセット
uspto-grants-1977_08
特許番号
US04040907

反応ノート

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