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L-Aspartic Acid → Pyruvic Acid
関与物質
| 役割 | 名称 | HCID | 仕込み量 |
|---|---|---|---|
| 反応物 | L-Aspartic Acid | HCID 5960 | — |
| 反応物 | Oxaloacetate Ion | HCID 3260017 | — |
| 反応物 | Magnesium Chloride | HCID 5360315 | — |
| 反応物 | 3-(1H-Indol-3-yl)-2-oxopropanoate | HCID 5460337 | — |
| 生成物 | Pyruvic Acid | HCID 1060 | — |
| 生成物 | L-Tryptophan | HCID 6305 | — |
反応手順
A solution of indole-3-pyruvate, 20 mM; L-aspartic acid 20 mM; MgCl2, 1.5 mM; transaminase, 0.3 mg/ml; oxaloacetate decarboxylase, 0.3 mg/ml buffered to pH 7.0 with 5 mM tris-hydroxymethylaminomethane hydrochloride (Tris) was stirred slowly for 2 hours. At the end of this time the reaction is complete. The L-tryptophan and pyruvic acid produced can be purified by methods well known in the art.
情報源と証拠
| DOI | 10.6084/m9.figshare.5104873.v1 |
|---|---|
| 特許番号 | US04518692 |
| 出典データセット | uspto-grants-1985_05 |
| ORD ID | ord-d77010d4b6754d73bea1dc6367e80517 |
| 出典メモ | uspto-grants-1985_05 |