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Udp-Glucose → 3'-Azido-2',3'-Dideoxyuridine 5-O-Diphosphoglucose
関与物質
| 役割 | 名称 | HCID | 仕込み量 |
|---|---|---|---|
| 反応物 | Udp-Glucose | HCID 8629 | — |
| 反応物 | Perchloric Acid | HCID 24247 | — |
| 反応物 | alpha-D-glucose 1-phosphate | HCID 65533 | — |
| 反応物 | Tromethamine hydrochloride | HCID 93573 | — |
| 反応物 | Magnesium Chloride | HCID 5360315 | — |
| 反応物 | AzddU triphosphate | HCID 131717599 | — |
| 生成物 | 3'-Azido-2',3'-Dideoxyuridine 5-O-Diphosphoglucose | HCID 131717598 | — |
反応手順
To 150 milligrams of AzddU-triphosphate was added 400 milligrams of glucose-1-phosphate, 6 millimole of MgCl2, 0.70 millimole of Tris-HCl pH 7.6, bacterial UDPG-pyrophosphorylase and inorganic pyrophosphatase. After 4 hours at room temperature, the reaction was stopped with perchloric acid (7N). After 30 minutes in ice, samples were centrifuged and the supernatant was neutralized with base. After another 30 minutes in ice, samples were recentrifuged and AzddU-5'-diphosphoglucose was purified by column chromatography.
情報源と証拠
| DOI | 10.6084/m9.figshare.5104873.v1 |
|---|---|
| 特許番号 | US05159067 |
| 出典データセット | uspto-grants-1992_10 |
| ORD ID | ord-3cfdeae288a2409fb130096d830f42b3 |
| 出典メモ | uspto-grants-1992_10 |