化工社化工社 化学情報印刷レポート · 研究参考用
Sodium Chloride → Deoxycholic Acid
関与物質
| 役割 | 名称 | HCID | 仕込み量 |
|---|---|---|---|
| 反応物 | Sodium Chloride | HCID 5234 | — |
| 反応物 | sodium (4R)-4-[(3R,5R,8R,9R,10S,12S,13R,14S,17R)-3,12-dihydroxy-8,10,13-trimethyl-1,2,3,4,5,6,7,9,11,12,14,15,16,17-tetradecahydrocyclopenta[a]phenanthren-17-yl]pentanoate | HCID 44582061 | — |
| 溶媒 | Tromethamine hydrochloride | HCID 93573 | — |
| 生成物 | Deoxycholic Acid | HCID 222528 | — |
反応手順
A pellet containing recombinant prothrombin immobilized on Ca3 (PO4)2 was obtained from a prothrombin-containing cell culture, as described above. Five 0.6 gram samples of moist adsorbate were suspended in 3 ml of 20 mM Tris-HCl buffer (pH 8.3) containing 50 mM, 100 mM, 250 mM, 350 mM or 500 mM sodium deoxycholate. Sixty microliter aliquots of 0.76 mg/ml trypsin were added to each sample, and the mixtures were incubated for three hours at room temperature with shaking. After incubation, the buffers of the samples were changed against 0.9% NaCl.
情報源と証拠
| DOI | 10.6084/m9.figshare.5104873.v1 |
|---|---|
| 特許番号 | US05792623 |
| 出典データセット | uspto-grants-1998_08 |
| ORD ID | ord-ea5a9a7ae3ab403ea5a1905e0b16be7b |
| 出典メモ | uspto-grants-1998_08 |