Carbon Tetrachloride → (S)-3-(3-(4-(5-amino-1,3,4-thiadiazol-2-yl)phenyl)pyrazolo[1,5-a]pyrimidin-5-yl)-4-isopropyloxazolidin-2-one

HRID 539002ノート

反応式

HRID 539002 の反応式
[CH:1]([C@H:4]1[CH2:8][O:7][C:6](=[O:9])[N:5]1[C:10]1[CH:15]=[CH:14][N:13]2[N:16]=[CH:17][C:18]([C:19]3[CH:31]=[CH:30][C:22]([C:23]([NH:25][NH:26][C:27](=[S:29])[NH2:28])=O)=[CH:21][CH:20]=3)=[C:12]2[N:11]=1)([CH3:3])[CH3:2].C1C=CC(P(C2C=CC=CC=2)C2C=CC=CC=2)=CC=1.C(N(CC)CC)C.C(Cl)(Cl)(Cl)Cl>ClCCl.CCOC(C)=O>[NH2:28][C:27]1[S:29][C:23]([C:22]2[CH:30]=[CH:31][C:19]([C:18]3[CH:17]=[N:16][N:13]4[CH:14]=[CH:15][C:10]([N:5]5[C@@H:4]([CH:1]([CH3:3])[CH3:2])[CH2:8][O:7][C:6]5=[O:9])=[N:11][C:12]=34)=[CH:20][CH:21]=2)=[N:25][N:26]=1

参加物

7

反応物 4

HCID 5943Carbon TetrachlorideHCID 8471TriethylamineHCID 11776TriphenylphosphineHCID 86667822(S)-2-(4-(5-(4-isopropyl-2-oxooxazolidin-3-yl)pyrazolo[1,5-a]pyrimidin-3-yl)benzoyl)hydrazinecarbothioamide

生成物 1

HCID 67034573(S)-3-(3-(4-(5-amino-1,3,4-thiadiazol-2-yl)phenyl)pyrazolo[1,5-a]pyrimidin-5-yl)-4-isopropyloxazolidin-2-one収率 7.4%

試薬・触媒・溶媒 2

HCID 6344Dichloromethane溶媒HCID 8857Ethyl Acetate溶媒

反応条件

温度
50 °C

実験手順

To a solution of (S)-2-(4-(5-(4-isopropyl-2-oxooxazolidin-3-yl)pyrazolo[1,5-a]pyrimidin-3-yl)benzoyl)hydrazinecarbothioamide (20.2 mg, 0.029 mmol) in dichloromethane (3 ml) was added PPh3 (22.7 mg, 0.087 mmol), triethylamine (32 μL, 0.231 mmol), and CCl4 (13.3 mg, 0.087 mmol). The reaction mixture was heated to 50° C. for 3 hours. After cooling to ambient temperature the reaction mixture was dissolved in EtOAc, washed with water then brine, dried over MgSO4, and concentrated under reduced pressure. The crude was purified by silica gel flash column chromatography (0-5% 7N NH3-MeOH in dichloromethane). The product was then subjected to preparative plate chromatography (10% MeOH in dichloromethane) to afford 0.9 mg (7%) of (S)-3-(3-(4-(5-amino-1,3,4-thiadiazol-2-yl)phenyl)pyrazolo[1,5-a]pyrimidin-5-yl)-4-isopropyloxazolidin-2-one. 1H NMR (400 MHz, CDCl3) δ 8.60 (d, 1H), 8.42 (s, 1H), 8.08 (m, 3H), 7.76 (d, 2H), 4.95 (m, 1H), 4.46 (m, 2H), 2.85 (m, 1H), 1.10 (d, 3H), 0.95 (d, 3H); LCMS (APCI+) m/z 422.5 [M+H]+.

後処理

  1. temperatureAfter cooling to ambient temperature the reaction mixture
  2. washwashed with water
  3. dry with materialbrine, dried over MgSO4
  4. concentrationconcentrated under reduced pressure
  5. customThe crude was purified by silica gel flash column chromatography (0-5% 7N NH3-MeOH in dichloromethane)

出典と根拠

ORDレコード
ord-390cb25509ef4a378e6ae07799dc1be0
出典データセット
uspto-grants-2014_10
特許番号
US08865726B2

反応ノート

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