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D-Alanine → DOTA biotin
関与物質
| 役割 | 名称 | HCID | 仕込み量 |
|---|---|---|---|
| 反応物 | D-Alanine | HCID 71080 | — |
| 反応物 | N-Hydroxysuccinimide | HCID 80170 | — |
| 反応物 | 1,3-Propanediamine, N'-(ethylcarbonimidoyl)-N,N-dimethyl-, monohydrochloride | HCID 2723939 | — |
| 反応物 | Biotinyl-d-alanine | HCID 22863182 | — |
| 反応物 | DOTA aniline | HCID 131719895 | — |
| 生成物 | DOTA biotin | HCID 87364501 | — |
反応手順
The D-alanine-linked conjugate was prepared by first coupling D-alanine (Sigma Chemical Co.) to biotin-NHS ester. The resultant biotinyl-D-alanine was then activated with 1-(3-dimethylaminopropyl)-3-ethyl-carbodiimide hydrochloride (EDCI) and N-hydroxysuccinimide (NHS). This NHS ester was reacted in situ with DOTA-aniline to give the desired product which was purified by preparative HPLC.
情報源と証拠
| DOI | 10.6084/m9.figshare.5104873.v1 |
|---|---|
| 特許番号 | US05624896 |
| 出典データセット | uspto-grants-1997_04 |
| ORD ID | ord-394a9cef44a645b19fb3a225578207dd |
| 出典メモ | uspto-grants-1997_04 |