HCID 178476363、化合物 → 5-Amino-3-(2',3,5'-tri-O-acetyl-beta-D-ribofuranosyl)-3H-thiazolo[4,5-d]pyrimidin-2-one

HRID 549793

反応式

HRID 549793 の反応式
[NH2:1][C:2]1[NH:3][C:4](=S)[C:5]2[S:10][C:9](=[O:11])[N:8]([C@@H:12]3[O:24][C@H:23]([CH2:25][O:26][C:27](=[O:29])[CH3:28])[C@@H:18]([O:19][C:20](=[O:22])[CH3:21])[C@H:13]3[O:14][C:15](=[O:17])[CH3:16])[C:6]=2[N:7]=1>CC(C)=O.[Ni]>[NH2:1][C:2]1[N:3]=[CH:4][C:5]2[S:10][C:9](=[O:11])[N:8]([C@@H:12]3[O:24][C@H:23]([CH2:25][O:26][C:27](=[O:29])[CH3:28])[C@@H:18]([O:19][C:20](=[O:22])[CH3:21])[C@H:13]3[O:14][C:15](=[O:17])[CH3:16])[C:6]=2[N:7]=1

参加物

4

反応物 1

HCID 178476363HCID 178476363、化合物

生成物 1

HCID 116332675-Amino-3-(2',3,5'-tri-O-acetyl-beta-D-ribofuranosyl)-3H-thiazolo[4,5-d]pyrimidin-2-one収率 60.1%

試薬・触媒・溶媒 2

HCID 935Nickel触媒HCID 180Acetone溶媒

実験手順

A suspension of Raney® 2800 nickel (3 big spatula, pre-washed with H2O, MeOH and acetone) in acetone (50 mL) was stirred at refluxing for 1 h. Triacetate 77 (0.93 g, 2.03 mmol) was subsequently added into the above suspension at reflux. The mixture was stirred for 5 min, cooled to room temperature over 30 min. The reaction was quenched by bubbling H2S (g) into the mixture for 2 h. The resulting mixture was filtered through a short pad of Celite® and washed with EtOH. The filtrate was concentrated and purified by flash column chromatography (silica, MeOH/CHCl3=1-2%) to afford 0.52 g (60%) of 78 as a white solid: mp 121-123° C.; 1H (400 MHz, d6-DMSO) δ 8.38 (s, 1H), 6.93 (s, 2H), 6.03 (d, J=3.6 Hz, 1H), 5.93 (dd, J=6.4, 3.6 Hz, 1H), 5.58 (t, J=6.0 Hz, 1H), 4.38 (dd, J=11.6, 3.6 Hz, 1H), 4.26 (m, 1H), 4.11 (q, J=6.0 Hz, 1H), 2.08 (s, 3H), 2.07 (s, 3H), 2.00 (s, 3H); MS (+)-ES [M+H]+ m/z 427.2. Elemental Analysis calc'd for C16H18N4O8S.0.5 CH3OH.0.25H2O: C, 44.34; H, 4.62; N, 12.54; S 7.17. Found: C, 44.54; H, 4.88; N, 12.16; S, 7.17.

後処理

  1. temperatureat refluxing for 1 h
  2. temperatureat reflux
  3. stirringThe mixture was stirred for 5 min
  4. customThe reaction was quenched
  5. customby bubbling H2S (g) into the mixture for 2 h
  6. filtrationThe resulting mixture was filtered through a short pad of Celite®
  7. washwashed with EtOH
  8. concentrationThe filtrate was concentrated
  9. custompurified by flash column chromatography (silica, MeOH/CHCl3=1-2%)

出典と根拠

ORDレコード
ord-5eb5447152274a60ab41ab3149e4dfe7
出典データセット
uspto-grants-2011_05
特許番号
US07943759B2

関連ノート

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