関与物質
| 役割 | 名称 | HCID | 仕込み量 |
|---|
| 反応物 | Pyruvate | HCID 107735 | — |
| 反応物 | Demannose | HCID 161658 | — |
| 反応物 | N-Acetyl-Neuraminic Acid | HCID 439197 | — |
| 反応物 | D-1,4-Dithiothreitol | HCID 446094 | — |
| 溶媒 | Potassium Phosphate, Tribasic | HCID 62657 | — |
| 生成物 | 3-Deoxy-L-glycero-L-galacto-2-nonulosonic acid | HCID 86749067 | 78% |
反応手順
To a 10 mL potassium phosphate buffer (0.1 M, pH 7.5) were added 10 mM DTT, 0.5 M pyruvate, 0.1 M D-mannose and 1.5 mg NeuAc aldolase. The reaction mixture was shaken at 37° C. for three days. The reaction mixture was chromatographed with a Dowex-1 (HCO3−) resin, eluted with zero to 1 M ammonium bicarbonate gradient. The fractions containing KDN were pooled and lyophilized three times repeatedly to remove the volatile salt to give a 78 percent yield of KDN. The physical data of the product were are consistent with the reported.
情報源と証拠
| DOI | 10.6084/m9.figshare.5104873.v1 |
|---|
| 特許番号 | US06168934A |
|---|
| 出典データセット | uspto-grants-2001_01 |
|---|
| ORD ID | ord-8c7478989ffc47cbb8dbdb7a7938d4f1 |
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| 出典メモ | uspto-grants-2001_01 |
|---|
